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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PerCP/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H6

Concentration

5 mg/ml

Product Specifications

Immunogen

Tissue / cell preparation. IL3R transfected COS cells.

Localization

Type I membrane protein.

Specificity

IL3RA (6H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5] [NBP2-00468] - Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) Alexa Fluor 647 and Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (NBP1-42839) (left) or Anti-Human CD123 PerCP-Cy5.5 (right). Cells in the monocyte gate were used for analysis.

Applications for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-3R alpha

The 6H6 monoclonal antibody reacts with human CD123, the a chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Gene Symbol

IL3RA

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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