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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G9

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.

Specificity

This antibody is specific for Homo sapiens interleukin 6 (interferon, beta 2) (IL6).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-6 Antibody (OTI3G9)

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810]

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810]

Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (3G9) [NBP1-47810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL6.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.

Applications for IL-6 Antibody (OTI3G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 5 using NBP1-47810 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-6

IL6 is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA (IL6R; MIM 147880), and the shared signaling receptor gp130 (IL6ST; MIM 600694)

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 3569 (Human)

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for IL-6 Antibody (OTI3G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-6 Antibody (OTI3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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