Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all IL-6 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-6 Antibody (OTI3G9) - Azide and BSA Free

Western Blot: IL-6 Antibody (OTI3G9)Azide and BSA Free [NBP2-71027]

Western Blot: IL-6 Antibody (OTI3G9)Azide and BSA Free [NBP2-71027]

Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 antibody (2G3) at 1:2000
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.
View 14 more images

Applications for IL-6 Antibody (OTI3G9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:50000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-6

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for IL-6 Antibody (OTI3G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-6 Antibody (OTI3G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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