Integrin alpha 4/CD49d Antibody (44H6)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64903

Novus Biologicals, part of Bio-Techne
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NB100-64903
NB100-64903-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 44H6

Concentration

1.0 mg/ml

View all Integrin alpha 4/CD49d Antibodies »

Product Specifications

Immunogen

HOON pre-B leukaemia cell line

Specificity

NB100-64903 recognizes CD49d, also known as Integrin alpha 4, which associates with either CD29 (integrin beta 1) or integrin beta 7. CD49d is a 150kD molecule, which may be cleaved to 80kD and 70kD fragments. It is expressed by lymphocytes, monocytes, eosinophils and thymocytes. VLA-4 mediates cell adhesion to VCAM-1 and fibronectin. 44H6 induces homotypic aggregation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin alpha 4/CD49d Antibody (44H6)

Flow Cytometry: Integrin alpha 4/CD49d Antibody (44H6) - BSA Free [NB100-64903]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (44H6) - BSA Free [NB100-64903]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (44H6) [NB100-64903] - Analysis using the FITC conjugate of NB100-64903. Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD49d: FITC.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (44H6) - BSA Free [NB100-64903]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (44H6) - BSA Free [NB100-64903]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (44H6) [NB100-64903] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD49d: RPE.

Applications for Integrin alpha 4/CD49d Antibody (44H6)

Application
Recommended Usage

Flow Cytometry

1:40

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:50-1:100
Application Notes
Overnight incubation with clone 44H6 is recommended for optimal results. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 4/CD49d

Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

UniProt

P13612

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (44H6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha 4/CD49d Antibody (44H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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