NB100-64903 recognizes CD49d, also known as Integrin alpha 4, which associates with either CD29 (integrin beta 1) or integrin beta 7. CD49d is a 150kD molecule, which may be cleaved to 80kD and 70kD fragments. It is expressed by lymphocytes, monocytes, eosinophils and thymocytes. VLA-4 mediates cell adhesion to VCAM-1 and fibronectin. 44H6 induces homotypic aggregation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Integrin alpha 4/CD49d

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Alternate Names

CD49d, ITGA4, VLA-4

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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Flow Cytometry

Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64903PEATT594

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all Integrin alpha 4/CD49d Antibodies »

Product Summary for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

Flow Cytometry

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin (Negative)

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Integrin alpha 4/CD49d

Alternate Names

Gene Symbol

Additional Integrin alpha 4/CD49d Products

Product Documents for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Integrin alpha 4/CD49d Antibody (44H6) [PE/Atto594]

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