Integrin alpha E/CD103 Antibody (ITGAE/3904R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07530

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ITGAE/3904R

Concentration

0.2 mg/ml

View all Integrin alpha E/CD103 Antibodies »

Product Summary for Integrin alpha E/CD103 Antibody (ITGAE/3904R)

Immunogen

Recombinant human Integrin alpha E/CD103 protein fragment (aa 775-855) (exact sequence is proprietary) (Uniprot: P38570 )

Localization

Cell surface and cytoplasm

Specificity

This monoclonal antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).

Marker

T-Cell Lymphoma & Hairy Cell Leukemia Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08855)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Integrin alpha E/CD103 Antibody (ITGAE/3904R)

Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) [NBP3-07530]

Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) [NBP3-07530]

Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) [NBP3-07530] - Formalin-fixed, paraffin-embedded human Tonsil stained with Integrin alpha E/CD103 Recombinant Rabbit Monoclonal Antibody (ITGAE/3904R).

Applications for Integrin alpha E/CD103 Antibody (ITGAE/3904R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Integrin alpha E/CD103

CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+.cells.

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

UniProt

P38570 (Human)

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (ITGAE/3904R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha E/CD103 Antibody (ITGAE/3904R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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