Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08855
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855]](https://resources.bio-techne.com/images/products/Integrin-alpha-E-CD103-Antibody-ITGAE-3904R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08855-img0001.jpg "Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ITGAE/3904R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Immunogen
Recombinant human Integrin alpha E/CD103 protein fragment (aa 775-855) (exact sequence is proprietary) (Uniprot: P38570 )
Localization
Cell surface and cytoplasm
Specificity
This monoclonal antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).
Marker
T-Cell Lymphoma & Hairy Cell Leukemia Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07530).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855]
Immunohistochemistry-Paraffin: Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free [NBP3-08855] - Formalin-fixed, paraffin-embedded human Tonsil stained with Integrin alpha E/CD103 Recombinant Rabbit Monoclonal Antibody (ITGAE/3904R).Applications for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Integrin alpha E/CD103
Alternate Names
CD103, HUMINAE, ITGAE
Gene Symbol
ITGAE
Additional Integrin alpha E/CD103 Products
Product Documents for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
Product Specific Notices for Integrin alpha E/CD103 Antibody (ITGAE/3904R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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