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Integrin alpha L/CD11a Antibody (I21/7) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-27872

Novus Biologicals, part of Bio-Techne
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NBP1-27872

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # I21/7

Concentration

0.5 mg/ml

Product Summary for Integrin alpha L/CD11a Antibody (I21/7) [Biotin]

Immunogen

Whole BW5147 cells

Specificity

Mouse/Human CD11a

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for Integrin alpha L/CD11a Antibody (I21/7) [Biotin]

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha L/CD11a

The monoclonal antibody I21/7 reacts with the alpha subunit of mouse CD11a, also known as Leukocyte Function-associated Antigen-1 (LFA-1) and a member of the integrin family of cell adhesion molecules. (1, 2) The antigen is expressed on thymocytes, T and B lymphocytes, and on non-lymphoid cells in the bone marrow. (2) CD11a appears to be an essential component for T-cell activation by antigen-presenting cells and is important for cell-mediated killing. Co-stimulatory signaling by LFA-1 is achieved by pairing with its ligand, CD54/ICAM-1. (1, 2)

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Gene Symbol

ITGAL

Additional Integrin alpha L/CD11a Products

Product Documents for Integrin alpha L/CD11a Antibody (I21/7) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha L/CD11a Antibody (I21/7) [Biotin]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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