Integrin beta 1/CD29 Antibody (12G10) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63255B

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB100-63255

Alexa Fluor 350

NB100-63255AF350

Alexa Fluor 405

NB100-63255AF405

Alexa Fluor 488

NB100-63255AF488

Alexa Fluor 532

NB100-63255AF532

Alexa Fluor 594

NB100-63255AF594

Alexa Fluor 647

NB100-63255AF647

Alexa Fluor 700

NB100-63255AF700

Alexa Fluor 750

NB100-63255AF750

Allophycocyanin

NB100-63255APC

Allophycocyanin/Cy7

NB100-63255APCCY7

CoraFluor 1

NB100-63255CL1

DyLight 350

NB100-63255UV

DyLight 405

NB100-63255V

DyLight 488

NB100-63255G

DyLight 550

NB100-63255R

DyLight 594

NB100-63255DL594

DyLight 650

NB100-63255C

DyLight 680

NB100-63255FR

DyLight 755

NB100-63255IR

FITC

NB100-63255F

HRP

NB100-63255H

Janelia Fluor 525

NB100-63255JF525

Janelia Fluor 549

NB100-63255JF549

Janelia Fluor 646

NB100-63255JF646

Janelia Fluor 669

NB100-63255JF669

mFluor Violet 450 SE

NB100-63255MFV450

mFluor Violet 500 SE

NB100-63255MFV500

mFluor Violet 610 SE

NB100-63255MFV610

PE

NB100-63255PE

PE/Atto594

NB100-63255PEATT594

PE/Cy5.5

NB100-63255PECY55

PE/Cy7

NB100-63255PECY7

PerCP

NB100-63255PCP

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 12G10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract

Epitope

Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.

Reactivity Notes

Predicted cross-reactivities: Mink, Rabbit

Localization

Type 1 membrane protein

Specificity

NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Integrin beta 1/CD29 Antibody (12G10) [Biotin]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (12G10) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Integrin beta 1/CD29 Antibody (12G10) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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