Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-63255CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 12G10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
Epitope
Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.
Reactivity Notes
Predicted cross-reactivities: Mink, Rabbit
Localization
Type 1 membrane protein
Specificity
NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.
Marker
Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
Additional Integrin beta 1/CD29 Products
Product Documents for Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]
Product Specific Notices for Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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