Integrin beta 1/CD29 Antibody (12G10)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63255

Novus Biologicals, part of Bio-Techne
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NB100-63255
NB100-63255-0.025mg
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 12G10

Concentration

1.0 mg/ml

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract

Epitope

Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.

Reactivity Notes

Predicted cross-reactivities: Mink, Rabbit

Localization

Type 1 membrane protein

Specificity

NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.

Marker

Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Integrin beta 1/CD29 Antibody (12G10)

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.
Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.
View 1 more images

Applications for Integrin beta 1/CD29 Antibody (12G10)

Application
Recommended Usage

ELISA

10 ug/mL

Flow Cytometry

1:25-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Reviewed Applications

Read 1 review rated 5 using NB100-63255 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human)

Gene Symbol

ITGB1

UniProt

P05556

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (12G10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin beta 1/CD29 Antibody (12G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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