Integrin beta 1/CD29 Antibody (12G10)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-63255
![Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-12G10-Flow-Cytometry-NB100-63255-img0002.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]")
![Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Knockdown Validated
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 12G10
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
Epitope
Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.
Localization
Type 1 membrane protein
Specificity
NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.
Marker
Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Integrin beta 1/CD29 Antibody (12G10)
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.![Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-12G10-Flow-Cytometry-NB100-63255-img0003.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]")
Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]
Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.![Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]")
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.Applications for Integrin beta 1/CD29 Antibody (12G10)
Application
Recommended Usage
ELISA
10 ug/mL
Flow Cytometry
1:25-1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB100-63255 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin beta 1/CD29
Additional Integrin beta 1/CD29 Products
Product Documents for Integrin beta 1/CD29 Antibody (12G10)
Product Specific Notices for Integrin beta 1/CD29 Antibody (12G10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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