Integrin beta 1/CD29 Antibody (12G10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-63255
![Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255] Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0005.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Knockdown Validated
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 12G10
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
Epitope
Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.
Reactivity Notes
Predicted cross-reactivities: Mink, Rabbit
Localization
Type 1 membrane protein
Specificity
NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.
Marker
Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Integrin beta 1/CD29 Antibody (12G10) - BSA Free
Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]
Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.Applications for Integrin beta 1/CD29 Antibody (12G10) - BSA Free
Application
Recommended Usage
ELISA
10 ug/mL
Flow Cytometry
1:25-1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Reviewed Applications
Read 1 review rated 5 using NB100-63255 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin beta 1/CD29
Additional Integrin beta 1/CD29 Products
Product Documents for Integrin beta 1/CD29 Antibody (12G10) - BSA Free
Product Specific Notices for Integrin beta 1/CD29 Antibody (12G10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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