Integrin beta 1/CD29 Antibody (P4C10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36561

Novus Biologicals, part of Bio-Techne
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NBP2-36561
NBP2-36561SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P4C10

Format

BSA Free

Concentration

1.0 mg/ml

View all Integrin beta 1/CD29 Antibodies »

Product Specifications

Immunogen

HT1080 cells human fibrosarcoma

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32803867). Mouse reactivity reported in scientific literature (PMID: 27550917). .

Specificity

Epitope is not trypsin sensitive.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]
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Applications for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunohistochemistry-Frozen

1:10-1:100

Western Blot

reported in scientific literature (PMID 27550917)
Application Notes
For ICC/IF ,methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. This product does contain sodium azide, so we highly recommend performing a buffer exchange or dialyzation prior to incubating with live cells.

Reviewed Applications

Read 1 review rated 4 using NBP2-36561 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms complexes with at least 18 alpha and 8 beta subunits in mammals. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Integrins can be found on the cell membrane, melanosome, cleavage furrows, and lamellipodium on a subcellular level. Expression levels in tissues are dependent on the isoform.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human)

Gene Symbol

ITGB1

UniProt

P05556

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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