Integrin beta 1/CD29 Antibody (P4C10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36561
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0003.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]")
![Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P4C10
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
HT1080 cells human fibrosarcoma
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:32803867). Mouse reactivity reported in scientific literature (PMID: 27550917). .
Specificity
Epitope is not trypsin sensitive.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0005.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]")
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0004.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - BSA Free [NBP2-36561]")
Applications for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10
Immunocytochemistry/ Immunofluorescence
1:10-1:100
Immunohistochemistry-Frozen
1:10-1:100
Western Blot
reported in scientific literature (PMID 27550917)
Application Notes
For ICC/IF ,methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. This product does contain sodium azide, so we highly recommend performing a buffer exchange or dialyzation prior to incubating with live cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-36561 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin beta 1/CD29
Additional Integrin beta 1/CD29 Products
Product Documents for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free
Product Specific Notices for Integrin beta 1/CD29 Antibody (P4C10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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