Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80818
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0003.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0005.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]")
![Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80818-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Block/Neutralize, CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P4C10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free
Immunogen
HT1080 cells human fibrosarcoma
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27550917).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]
Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively. Image from the standard format of this antibody.![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0004.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]")
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]](https://resources.bio-techne.com/images/products/Integrin-beta-1-CD29-Antibody-P4C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80818-img0002.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]")
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818]
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free [NBP2-80818] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.Applications for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10
Immunocytochemistry/ Immunofluorescence
1:10-1:100
Immunohistochemistry-Frozen
1:10-1:100
Western Blot
reported in scientific literature (PMID 27550917)
Application Notes
For ICC/IF ,methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
Additional Integrin beta 1/CD29 Products
Product Documents for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free
Product Specific Notices for Integrin beta 1/CD29 Antibody (P4C10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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