Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29800
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Primate, Rabbit
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CSbeta6
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
beta 6 knockout mice have been immunized with recombinant secreted human alpaVbeta6.
Specificity
Monoclonal antibody MAB2076Z, raised by immunizing beta6 knockout mice with recombinant secreted human alphavbeta6, is useful for flow cytometry and immunoprecipitation in all species tested, including mouse. It also works well for immunohistochemistry of acetone-fixed tissues in several species, including rat, rabbit, monkey, and human. The antibody does not work well for immunohistochemistry of mouse tissue. This antibody does not recognize the aV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western Blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Relevant Entrez Gene Numbers: NM_000888.3
Applications for Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
Immunohistochemistry (acetone fixation): Effective for Rabbit, rat, monkey, and human. Not effective for mouse tissue. Immunocytochemistry/Immunofluorescence : Effective for F6, HT-29, H441 cell lines (others not tested). Flow cytometry: Effective for all species tested, including rabbit, rat, monkey, human, and mouse. Immunoprecipitation
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Integrin beta 6
Additional Integrin beta 6 Products
Product Documents for Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free
Product Specific Notices for Integrin beta 6 Antibody (CSbeta6) - Azide and BSA Free
Relevant Entrez Gene Numbers: NM_000888.3
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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