Jagged 1 Antibody (1E12)

Western Blot: Jagged 1 Antibody (1E12) [H00000182-M01A]

Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .

Knockout Validated: Jagged 1 Antibody (1E12) [H00000182-M01A]

Knockout Validated: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged 1 regulates learning-dependent Notch induction and is enriched presynaptically. Fluorescent double-labeling shows the expression of Notch1 (red) following spatial exploration in CTLs and Jagged 1cKO. Jagged 1 labeling (green) is used to validate the absence of Jagged 1 in the Jagged 1cKO (Jag1cKOs). Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fncel.2017.00220/full) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] -

Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Targeted loss of Jagged1 in adult mouse neurons causes a spatial memory deficit. A schematic representation of the Jagged1 floxed allele used to generate the mice with TAM-inducible loss of Jagged1 gene (A). Representative immunofluorescence images of single confocal z-plane showing a near complete loss of Jagged1 protein (green) from hippocampal pyramidal neurons labeled by NeuN (red) of Jagged1cKO mice (B). Representative images from immunoblots showing the expression of Jagged1, DNER in Jagged1cKO mice & control mice (C). GAPDH is used as a loading control. Bar graph showing the quantitation of optical densities of Jagged1 & DNER bands in CTLs & Jagged1cKO mice (D). A graphic showing the behavioral arena for Y-maze spontaneous alternation test (E). Jagged1cKO mice show a significant deficit in hippocampus dependent working memory in the Y-maze spontaneous alternation test (F). A diagram showing the experimental arena for the hidden arm version of the Y-maze (G). Jagged1cKO mice show a significant reduction in the time spent in the hidden arm, suggesting a spatial memory defect (H). A schematic showing the experimental setup for the Novel Object Displacement test (I). Jagged1cKO mice exhibit a statistical significant reduction in discrimination index, a measure of spatial memory defect (J). Scale bar in (B) is 50 μm. Graphs are represented as mean ± SEM, *p < 0.05, **p < 0.01, & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.