JNK1 Antibody (1E5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22209

Novus Biologicals, part of Bio-Techne
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NBP2-22209-0.025ml
NBP2-22209
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E5

Format

BSA Free

Concentration

1 mg/ml

View all JNK1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human JNK1 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JNK1 Antibody (1E5) - BSA Free

Western Blot: JNK1 Antibody (1E5)BSA Free [NBP2-22209]

Western Blot: JNK1 Antibody (1E5)BSA Free [NBP2-22209]

Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using JNK1 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.
Flow Cytometry: JNK1 Antibody (1E5) - BSA Free [NBP2-22209]

Flow Cytometry: JNK1 Antibody (1E5) - BSA Free [NBP2-22209]

Flow Cytometry: JNK1 Antibody (1E5) [NBP2-22209] - Flow cytometric analysis of HeLa cells using JNK1 mouse mAb (green) and negative control (red).
Western Blot: JNK1 Antibody (1E5)BSA Free [NBP2-22209]

Western Blot: JNK1 Antibody (1E5)BSA Free [NBP2-22209]

Western Blot: JNK1 Antibody (1E5) [NBP2-22209] - Western blot analysis using MAPK8 mAb against human JNK1 (AA: 227-380) recombinant protein. (Expected MW is 43.4 kDa)
View 1 more images

Applications for JNK1 Antibody (1E5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JNK1

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Gene Symbol

MAPK8

Additional JNK1 Products

Product Documents for JNK1 Antibody (1E5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JNK1 Antibody (1E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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