JNK1 Antibody (2F11)

Novus Biologicals, part of Bio-Techne | Catalog # H00005599-M11

Novus Biologicals, part of Bio-Techne
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H00005599-M11
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all JNK1 Antibodies »

Product Specifications

Immunogen

MAPK8 (NP_002741.1, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLAQVQQ

Specificity

MAPK8 - mitogen-activated protein kinase 8 (2F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for JNK1 Antibody (2F11)

Western Blot: JNK1 Antibody (2F11) [H00005599-M11]

Western Blot: JNK1 Antibody (2F11) [H00005599-M11]

Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11]

Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11]

Immunocytochemistry/Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of monoclonal antibody to MAPK8 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: JNK1 Antibody (2F11) [H00005599-M11]

Western Blot: JNK1 Antibody (2F11) [H00005599-M11]

Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi or non-transfected control. Blot probed with H00005599-M11. GAPDH (36.1 kDa) used as loading control.
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Applications for JNK1 Antibody (2F11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: JNK1

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Entrez Gene IDs

5599 (Human)

Gene Symbol

MAPK8

UniProt

NP_002741

Additional JNK1 Products

Product Documents for JNK1 Antibody (2F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JNK1 Antibody (2F11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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