JNK1 [p Thr183, p Tyr185] Antibody (HL1008)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13678
Recombinant Monoclonal Antibody
![JNK1 [p Thr183, p Tyr185] Antibody (HL1008)](https://resources.bio-techne.com/images/products/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-9102024204127.jpg "Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -")
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1008
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr183/Tyr185 of human JNK1. The exact sequence is proprietary.
Modification
p Thr183, p Tyr185
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for JNK1 [p Thr183, p Tyr185] Antibody (HL1008)
Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - JNK1 (phospho Thr183/Tyr185) antibody [HL1008] detects JNK1 (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. JNK1 (phospho Thr183/Tyr185) stained by JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -
Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 (phospho Thr183/Tyr185) antibody [HL1008] (NBP3-13678) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![JNK1 [p Thr183, p Tyr185] Antibody (HL1008)](https://resources.bio-techne.com/images/products/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020242013406.jpg "Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -")
![JNK1 [p Thr183, p Tyr185] Antibody (HL1008)](https://resources.bio-techne.com/images/products/nbp3-13678_rabbit-jnk1-p-thr-183-p-tyr-185-mab-hl1008-91020241959351.jpg "Western Blot: JNK1 [p Thr183, p Tyr185] Antibody (HL1008) [NBP3-13678] -")
Applications for JNK1 [p Thr183, p Tyr185] Antibody (HL1008)
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: JNK1
Long Name
C-Jun N-terminal Kinase 1
Alternate Names
MAPK8, PRKM8, SAPK1
Gene Symbol
MAPK8
Additional JNK1 Products
Product Documents for JNK1 [p Thr183, p Tyr185] Antibody (HL1008)
Product Specific Notices for JNK1 [p Thr183, p Tyr185] Antibody (HL1008)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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