Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mustelid, Primate

Applications

Chromatin Immunoprecipitation (ChIP), Electron Microscopy, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RW105

Format

Azide and BSA Free

Concentration

1 mg/ml

View all p300 Antibodies »

Product Summary for KAT3B/p300 Antibody (RW105) - Azide and BSA Free

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]

Reactivity Notes

Mink.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for KAT3B/p300 Antibody (RW105) - Azide and BSA Free

Western Blot: KAT3B/p300 Antibody (RW105)Azide and BSA Free [NBP2-80820]

Western Blot: KAT3B/p300 Antibody (RW105)Azide and BSA Free [NBP2-80820]

Western Blot: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820]

Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820]

Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW105) NBP2-80820 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820]

Flow Cytometry: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820]

Flow Cytometry: KAT3B/p300 Antibody (RW105) - Azide and BSA Free [NBP2-80820] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin. Image using the PE format of this antibody. Image from the standard format of this antibody.
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Applications for KAT3B/p300 Antibody (RW105) - Azide and BSA Free

Application
Recommended Usage

Electron Microscopy

reported in scientific literature (PMID 25728767)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:10-1:500

In vitro assay

reported in scientific literature (PMID 25728767)

Western Blot

1:250-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p300

In the eukaryotic genome, chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Long Name

E1A-binding Protein p300

Alternate Names

EP300

Gene Symbol

EP300

Additional p300 Products

Product Documents for KAT3B/p300 Antibody (RW105) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KAT3B/p300 Antibody (RW105) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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