KAT3B/p300 Antibody (RW109)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-617

Novus Biologicals, part of Bio-Techne
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NB100-617
NB100-617SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Mustelid, Primate

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RW109

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all p300 Antibodies »

Product Specifications

Immunogen

Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q09472]

Epitope

The epitope recognized is found between amino acids 2107-2283.

Reactivity Notes

Mink.

Localization

Nuclear

Specificity

This is specific for p300 protein. This recognizes residues 2107-2283.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

300 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KAT3B/p300 Antibody (RW109)

Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617]

Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617]

Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.

Applications for KAT3B/p300 Antibody (RW109)

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
In Western Blot, a band is seen at ~300 kDa. A lower non-specific MW bands (~50 kDa) may be seen with longer exposure times.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p300

In eukaryotic genome, the chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and it is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Long Name

E1A-binding Protein p300

Alternate Names

EP300

Entrez Gene IDs

2033 (Human); 328572 (Mouse); 170915 (Rat)

Gene Symbol

EP300

Additional p300 Products

Product Documents for KAT3B/p300 Antibody (RW109)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KAT3B/p300 Antibody (RW109)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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