KLF4 Antibody (CL5785)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61427

Novus Biologicals, part of Bio-Techne
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NBP2-61427
NBP2-61427-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5785

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all KLF4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: GREKTLRQAGAPNNRWREE

Reactivity Notes

Mouse, Rat (89%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KLF4 Antibody (CL5785)

Western Blot: KLF4 Antibody (CL5785) [NBP2-61427]

Western Blot: KLF4 Antibody (CL5785) [NBP2-61427]

Western Blot: KLF4 Antibody (CL5785) [NBP2-61427] - Analysis in human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427]

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of U-2197cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427]

Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).
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Applications for KLF4 Antibody (CL5785)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

Additional KLF4 Products

Product Documents for KLF4 Antibody (CL5785)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for KLF4 Antibody (CL5785)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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