KLF4 Antibody (CL5785) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44208
![KLF4 Antibody (CL5785) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-28220251339191.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]")
![KLF4 Antibody (CL5785) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-28220251232107.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL5785
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of O43474, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for KLF4 Antibody (CL5785) - Azide and BSA Free
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]
Staining of human endometrium shows nuclear immunoreactivity in glandular and stromal cells.![KLF4 Antibody (CL5785) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-282202512495834.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]")
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]
Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).![KLF4 Antibody (CL5785) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-28220251324521.jpg "Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP3-44208]")
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP3-44208]
Staining of PC-3 cells using the Anti-KLF4 monoclonal antibody) .![KLF4 Antibody (CL5785) - Azide and BSA Free Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-282202513254779.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP3-44208]")
![KLF4 Antibody (CL5785) - Azide and BSA Free Western Blot: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-282202513261926.jpg "Western Blot: KLF4 Antibody [NBP3-44208]")
![KLF4 Antibody (CL5785) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP3-44208]](https://resources.bio-techne.com/images/products/nbp3-44208_mouse-klf4-mab-cl5785-azide-and-bsa-free-282202513314653.jpg "Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP3-44208]")
Applications for KLF4 Antibody (CL5785) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KLF4
Long Name
Kruppel-Like Factor 4
Alternate Names
EZF
Gene Symbol
KLF4
Additional KLF4 Products
Product Documents for KLF4 Antibody (CL5785) - Azide and BSA Free
Product Specific Notices for KLF4 Antibody (CL5785) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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