Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7F6

Format

BSA Free

Concentration

1 mg/ml

View all LAP (TGF-beta 1) Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human TGF beta 1 (amino acids 62-197) expressed in E. coli. [UniProt# P01137]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26851347). Rat reactivity reported in scientific literature (PMID: 30147399).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LAP (TGF-beta 1) Antibody (7F6) - BSA Free

Western Blot: LAP (TGF-beta 1) Antibody (7F6)BSA Free [NBP2-22114]

Western Blot: LAP (TGF-beta 1) Antibody (7F6)BSA Free [NBP2-22114]

Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) - BSA Free [NBP2-22114]

Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) - BSA Free [NBP2-22114]

Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.
Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) - BSA Free [NBP2-22114]

Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) - BSA Free [NBP2-22114]

Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).
View 4 more images

Applications for LAP (TGF-beta 1) Antibody (7F6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

reported in scientific literature (PMID 30147399)

Immunohistochemistry-Paraffin

reported by customer review

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-22114 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAP (TGF-beta 1)

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease

Long Name

Latency-associated Peptide

Alternate Names

LAP (TGFbeta 1)

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional LAP (TGF-beta 1) Products

Product Documents for LAP (TGF-beta 1) Antibody (7F6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LAP (TGF-beta 1) Antibody (7F6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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