LIF Antibody (39N7D10) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27406CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # 39N7D10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for LIF Antibody (39N7D10) [CoraFluor™ 1]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The LIF protein can be highly glycosylated and has been observed between 22-34 in Western Blotting. Staining in IHC-P is enhanced through antigen retrieval using 10 mM Sodium Citrate buffer, pH 6.0.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: LIF
- Fully Active
- -Fully folded naturally by eukaryotic source
- -Comparable for eukaryotic glycosylation
- BioRisk Free
- -animal-free
- -serum-free
- -endotoxin-free
- -infection reagent-free
- -low in proteolyic activity
- -low in pyrogenic and pro-inflammatory activity
- -serum-free
- Just reconstitute and add to your cell culture.
- Have FDA G.R.A.S. (generally recognized as safe) approval.
Long Name
Leukemia Inhibitory Factor
Alternate Names
D Factor, Emfilermin, HILDA, MLPLI
Gene Symbol
LIF
Additional LIF Products
Product Documents for LIF Antibody (39N7D10) [CoraFluor™ 1]
Product Specific Notices for LIF Antibody (39N7D10) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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