LIF Antibody (39N7D10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27406

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 39N7D10

Format

BSA Free

Concentration

1.0 mg/ml

View all LIF Antibodies »

Product Specifications

Immunogen

A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for LIF Antibody (39N7D10) - BSA Free

Western Blot: LIF Antibody (39N7D10)BSA Free [NBP2-27406]

Western Blot: LIF Antibody (39N7D10)BSA Free [NBP2-27406]

Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - BSA Free [NBP2-27406]

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - BSA Free [NBP2-27406]

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).
Western Blot: LIF Antibody (39N7D10)BSA Free [NBP2-27406]

Western Blot: LIF Antibody (39N7D10)BSA Free [NBP2-27406]

Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.
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Applications for LIF Antibody (39N7D10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

3-5ug/ml~
Application Notes
The LIF protein can be highly glycosylated and has been observed between 22-34 in Western Blotting. Staining in IHC-P is enhanced through antigen retrieval using 10 mM Sodium Citrate buffer, pH 6.0.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-27406 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIF

Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells. Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor), Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells. Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiated. This illustrates a different functional program for Lif in mice and humans in maintenance of stem cells. An IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

UniProt

P09056

Additional LIF Products

Product Documents for LIF Antibody (39N7D10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LIF Antibody (39N7D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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