LIF Antibody (39N7D10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80834

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 39N7D10

Format

Azide and BSA Free

Concentration

1 mg/ml

View all LIF Antibodies »

Product Specifications

Immunogen

A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for LIF Antibody (39N7D10) - Azide and BSA Free

Western Blot: LIF Antibody (39N7D10)Azide and BSA Free [NBP2-80834]

Western Blot: LIF Antibody (39N7D10)Azide and BSA Free [NBP2-80834]

Western Blot: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay. Image from the standard format of this antibody.
Immunocytochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834]

Immunocytochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834]

Immunocytochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Image from the standard format of this antibody.
Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834]

Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834]

Immunohistochemistry: LIF Antibody (39N7D10) - Azide and BSA Free [NBP2-80834] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules. Image from the standard format of thi
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Applications for LIF Antibody (39N7D10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

3 - 5 ug/ml
Application Notes
The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. Staining in IHC-P is enhanced through antigen retrieval using 10 mM Sodium Citrate buffer, pH 6.0.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIF

Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

Additional LIF Products

Product Documents for LIF Antibody (39N7D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LIF Antibody (39N7D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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