Skip to main content

Ly-6C Antibody (ER-MP20)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65413

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB100-65413
NB100-65413-0.025mg
Conjugate
Catalog #

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # ER-MP20

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Balb/c macrophage precursor cell hybrids

Specificity

Ly6C (ER-MP20)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Ly-6C Antibody (ER-MP20)

Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]

Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]

Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.
Flow Cytometry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]

Flow Cytometry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]

Flow Cytometry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE.
Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]

Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]

Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.

Applications for Ly-6C Antibody (ER-MP20)

Application
Recommended Usage

Flow Cytometry

1:10-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ly-6C

Ly-6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes. Using ER-MP20, distinct mouse blood monocyte subsets can be distinguished: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.

Alternate Names

Ly 6c, Ly6c protein, Lymphocyte antigen 6 complex, Lymphocyte antigen 6 complex locus C, Lymphocyte antigen 6C, Lymphocyte antigen Ly 6C

Entrez Gene IDs

17067 (Mouse)

Gene Symbol

Ly6c1

Additional Ly-6C Products

Product Documents for Ly-6C Antibody (ER-MP20)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ly-6C Antibody (ER-MP20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...