Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49602

Novus Biologicals, part of Bio-Techne
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NBP1-49602
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.53 mg/ml

View all Lysine (K)-specific Demethylase 4A/KDM4A Antibodies »

Product Summary for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Immunogen

A genomic peptide made to an internal region of the human JMJD2A protein (within residues 320-460). [Swiss-Prot O75164]

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A AntibodyBSA Free [NBP1-49602]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A AntibodyBSA Free [NBP1-49602]

Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free [NBP1-49602]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free [NBP1-49602]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free [NBP1-49602]

Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free [NBP1-49602]

Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Staining of JMJD2A in paraffin embedded mouse pancreas.
View 1 more images

Applications for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Simple Western

1:500

Western Blot

1:1000 - 1:2000
Application Notes
This JMJD2A antibody is useful for Immunohistochemistry on paraffin tissues, Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~119 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.53 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 4A/KDM4A

Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D) are Fe(II)- and alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2A (JHDM3 or KDM4A) catalyzes the demethylation of di- and trimethylated H3K9 and H3K36 and participate in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of HDACs and NCOR1, respectively. JMJD2A as well as JMJD2C have been suggested to act as co-activators of AR-dependent gene transcription. JMJD2A is widely expressed in human tissues and cell lines with high expression in human T-cell lymphotropic virus 1-infected cell lines, HT1376 bladder carcinoma cell line, U2OS osteosarcoma cell line and prostate cancer cell lines. JMJD2A is overexpressed in mouse and human lung cancers has been proposed as an oncogene that represents a target for Ras-expressing tumors. Silencing of JMJD2A gene has been shown to result in cell cycle change and proliferation inhibition, and suppression of tumor cell migration/ invasion.

Alternate Names

JHDM3A, JMJD2, JMJD2A, KDM4A, Lysine (K)specific Demethylase 4A

Entrez Gene IDs

9682 (Human); 230674 (Mouse)

Gene Symbol

KDM4A

UniProt

O75164 (Human)

Additional Lysine (K)-specific Demethylase 4A/KDM4A Products

Product Documents for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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