Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52969

Novus Biologicals, part of Bio-Techne
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NBP2-52969
NBP2-52969-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1129

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lysine (K)-specific Demethylase 5B/KDM5B Antibodies »

Product Summary for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE

Epitope

DGINSLERKLKRRLE

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (86%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129)

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of MCF7 cells using the Anti-KDM5B monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) [NBP2-52969] - Staining of human uterus shows moderate nuclear positivity in glandular cells.
View 6 more images

Applications for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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