Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43901

Novus Biologicals, part of Bio-Techne
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NBP3-43901
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NBP2-52969
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1129

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Lysine (K)-specific Demethylase 5B/KDM5B Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q9UGL1, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) - Azide and BSA Free Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-43901]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-43901]

Analysis in human testis and liver tissues using NBP3-43901 antibody. Corresponding Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B RNA-seq data are presented for the same tissues.
Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) - Azide and BSA Free Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-43901]

Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-43901]

Staining of human breast cancer shows moderate nuclear positivity in tumor cells.
Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody (CL1129) - Azide and BSA Free Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-43901]

Western Blot: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibody [NBP3-43901]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate) 
Lane 3: Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
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Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 5B/KDM5B

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.

Alternate Names

CT31, Jarid1B, KDM5B, Lysine (K)specific Demethylase 5B, PLU-1, PUT1, RBBP2H1

Gene Symbol

KDM5B

Additional Lysine (K)-specific Demethylase 5B/KDM5B Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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