MAP3K8/Tpl2/COT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14395

Novus Biologicals, part of Bio-Techne
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NBP3-14395
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bat (100%), Bovine (100%), Canine (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

0.1 mg/ml

View all MAP3K8/Tpl2/COT Antibodies »

Product Specifications

Immunogen

Synthetic 18 amino acid peptide near C-Terminus of human MAP3K8/Tpl2/COT.

Epitope

C-Terminus

Specificity

Human MAP3K8/Tpl2/COT. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for MAP3K8/Tpl2/COT Antibody

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395]

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395]

Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon after heat-induced antigen retrieval.

Applications for MAP3K8/Tpl2/COT Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: MAP3K8/Tpl2/COT

The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 8

Alternate Names

COT, EST, ESTF, MAP3K8, Tpl-2

Gene Symbol

MAP3K8

Additional MAP3K8/Tpl2/COT Products

Product Documents for MAP3K8/Tpl2/COT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP3K8/Tpl2/COT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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