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MEK1/2 Antibody (SR1751)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21632

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21632-100ul
NBP3-21632-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1751

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MEK1/2 Antibody (SR1751)

Immunogen

A synthesized peptide derived from human MEK1/2 (Uniprot #: Q02750, P36507)

Specificity

Detects endogenous levels of total MEK1/2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK1/2 Antibody (SR1751)

Western Blot: MEK1/2 Antibody (SR1751) [NBP3-21632] -

Western Blot: MEK1/2 Antibody (SR1751) [NBP3-21632] - Western blot analysis using NBP3-21632 in (1) 293T cell lysate; (2) A549 cell lysate.

Immunocytochemistry/Immunofluorescence: MEK1/2 Antibody (SR1751) [NBP3-21632] -

Immunocytochemistry/Immunofluorescence: MEK1/2 Antibody (SR1751) [NBP3-21632] - Immunofluorescent analysis of Hela cells, using NBP3-21632.

Immunohistochemistry: MEK1/2 Antibody (SR1751) [NBP3-21632] -

Immunohistochemistry: MEK1/2 Antibody (SR1751) [NBP3-21632] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21632.

Applications for MEK1/2 Antibody (SR1751)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:100-1:500

Immunoprecipitation

1:50-1:100

Western Blot

1:5000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEK1/MEK2

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Gene Symbol

MAP2K1

Additional MEK1/MEK2 Products

Product Documents for MEK1/2 Antibody (SR1751)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MEK1/2 Antibody (SR1751)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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