Melan-A/MART-1 Antibody (DT101 + BC199)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34246

Novus Biologicals, part of Bio-Techne
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NBP2-34246-0.02mg
NBP2-34246-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda/IgG2b Kappa Clone # DT101 + BC199

Concentration

0.2 mg/ml

View all Melan-A/MART-1 Antibodies »

Product Summary for Melan-A/MART-1 Antibody (DT101 + BC199)

Immunogen

Recombinant hMelan-A/MART-1 protein (DT101 & BC199) (Uniprot: Q16655)

Localization

Cytoplasmic

Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda/IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34607)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Melan-A/MART-1 Antibody (DT101 + BC199)

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) [NBP2-34246]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) [NBP2-34246]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101+ BC199) [NBP2-34246] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Mouse Monoclonal Antibody (DT101 + BC199).
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) [NBP2-34246]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) [NBP2-34246]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101+ BC199) [NBP2-34246] - Formalin-fixed, paraffin-embedded human melanoma stained withMelan-A MAb (DT101+BC199). Note cytoplasmic staining of cells.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) [NBP2-34246]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) [NBP2-34246]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101+ BC199) [NBP2-34246] - Human Melanoma sample was stained for MART-1 positive cells, in green and counter-stained for DAPI in blue. This image was submitted via customer Review.

Applications for Melan-A/MART-1 Antibody (DT101 + BC199)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34246 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

UniProt

Q16655

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (DT101 + BC199)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melan-A/MART-1 Antibody (DT101 + BC199)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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