Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34607

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda/IgG2b Kappa Clone # DT101 + BC199

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Melan-A/MART-1 Antibodies »

Product Specifications

Immunogen

Recombinant hMelan-A/MART-1 protein (DT101 & BC199) (Uniprot: Q16655)

Localization

Cytoplasmic

Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda/IgG2b Kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34246).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free [NBP2-34607]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free [NBP2-34607]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101+ BC199) - Azide and BSA Free [NBP2-34607] - Formalin-fixed, paraffin-embedded human melanoma stained with Melan-A MAb (DT101+BC199). Note cytoplasmic staining of cells.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free [NBP2-34607]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free [NBP2-34607]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (DT101+ BC199) - Azide and BSA Free [NBP2-34607] - Human Melanoma sample was stained for MART-1 positive cells, in green and counter-stained for DAPI in blue. This image was submitted via customer Review.

Applications for Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Melan-A/MART-1 Antibody (DT101 + BC199) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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