MHC Class I Antibody (MEM-E/08) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-305
![Flow Cytometry: MHC Class I Antibody (MEM-E/08) - BSA Free [NB500-305]](https://resources.bio-techne.com/images/products/MHC-Class-I-Antibody-MEM-E-08-Flow-Cytometry-NB500-305-img0001.jpg "Flow Cytometry: MHC Class I Antibody (MEM-E/08) - BSA Free [NB500-305]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-E/08
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for MHC Class I Antibody (MEM-E/08) - BSA Free
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Localization
Type I membrane protein.
Specificity
This antibody (clone MEM-E/08) recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MHC Class I Antibody (MEM-E/08) - BSA Free
Flow Cytometry: MHC Class I Antibody (MEM-E/08) - BSA Free [NB500-305]
Flow Cytometry: MHC Class I Antibody (MEM-E/08) [NB500-305] - Flow cytometry analysis (surface staining) of HLA-E-transfectants with anti-HLA-E (MEM-E/08) purified, GAM-APCApplications for MHC Class I Antibody (MEM-E/08) - BSA Free
Application
Recommended Usage
Flow Cytometry
4 ug/ml
Immunoprecipitation
1:50
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MHC Class I
Alternate Names
DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1
Entrez Gene IDs
3133 (Human)
Gene Symbol
HLA-E
Additional MHC Class I Products
Product Documents for MHC Class I Antibody (MEM-E/08) - BSA Free
Product Specific Notices for MHC Class I Antibody (MEM-E/08) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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