MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-305MFV450

Novus Biologicals, part of Bio-Techne
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NB500-305MFV450
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-E/08

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MHC Class I Antibodies »

Product Specifications

Immunogen

Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Localization

Type I membrane protein.

Specificity

This antibody (clone MEM-E/08) recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE]

MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE] [NB500-305MFV450] -

MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE] [NB500-305MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

Applications for MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MHC Class I

The antigenic agglomerate major histocompatibility complex (MHC) class I molecules present antigenic peptides to CD8-positive T cells. It is now recognized that the products of the MHC genes are antigen-presenting molecules (APM) designed for the presentation of antigen fragments (peptides) to the T-cell receptor, thus participating in the immune response (1). Recently shown is a mechanism of antigen acquisition for cross-presentation that couples the antigen presentation system of two adjacent cells and is lost in most tumours: gap-junction-mediated intercellular peptide coupling for presentation by bystander MHC class I molecules and transfer to professional antigen presenting cells for cross-priming (2). The mammalian vomeronasal organ detects social information about gender, status, and individuality. It has been shown that small peptides that serve as ligands for major histocompatibility complex (MHC) class I molecules function also as sensory stimuli for a subset of vomeronasal sensory neurons located in the basal Gao- and V2R receptor-expressing zone of the vomeronasal epithelium (3).

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MHC Class I Antibody (MEM-E/08) [mFluor Violet 450 SE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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