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MHC Class II Antibody (HIS19) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43606

Novus Biologicals, part of Bio-Techne
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NBP1-43606
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Key Product Details

Species Reactivity

Validated:

Rat

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # HIS19

Concentration

0.5 mg/ml

Product Summary for MHC Class II Antibody (HIS19) [Biotin]

Immunogen

The immunogen for this antibody is MHC Class II.

Reactivity Notes

Rat

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for MHC Class II Antibody (HIS19) [Biotin]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cell suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS19 clone has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MHC Class II

The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.

Alternate Names

class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM

Gene Symbol

HLA-DMA

Additional MHC Class II Products

Product Documents for MHC Class II Antibody (HIS19) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MHC Class II Antibody (HIS19) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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