MHC Class II Antibody (HIS19) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00462
![Flow Cytometry: MHC Class II Antibody (HIS19) - BSA Free [NBP2-00462]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-HIS19-Flow-Cytometry-NBP2-00462-img0002.jpg "Flow Cytometry: MHC Class II Antibody (HIS19) - BSA Free [NBP2-00462]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # HIS19
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody is MHC Class II.
Reactivity Notes
Rat
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MHC Class II Antibody (HIS19) - BSA Free
Flow Cytometry: MHC Class II Antibody (HIS19) - BSA Free [NBP2-00462]
Flow Cytometry: MHC Class II Antibody (HIS19) [NBP2-00462] - Analysis using the FITC conjugate of NBP2-00462. Staining of rat splenocytes with Anti-Rat MHC Class II FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.Applications for MHC Class II Antibody (HIS19) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry-Frozen
1:10-1:500
Application Notes
The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cell suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS19 clone has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MHC Class II
Alternate Names
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbol
HLA-DMA
Additional MHC Class II Products
Product Documents for MHC Class II Antibody (HIS19) - BSA Free
Product Specific Notices for MHC Class II Antibody (HIS19) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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