MMP-2 Antibody (8B4) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB200-114CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB200-114AF350

Alexa Fluor 405

NB200-114AF405

Alexa Fluor 488

NB200-114AF488

Alexa Fluor 532

NB200-114AF532

Alexa Fluor 594

NB200-114AF594

Alexa Fluor 647

NB200-114AF647

Alexa Fluor 700

NB200-114AF700

Alexa Fluor 750

NB200-114AF750

Biotin

NB200-114B

DyLight 350

NB200-114UV

DyLight 405

NB200-114V

DyLight 488

NB200-114G

DyLight 550

NB200-114R

DyLight 594

NB200-114DL594

DyLight 650

NB200-114C

DyLight 680

NB200-114FR

DyLight 755

NB200-114IR

FITC

NB200-114F

HRP

NB200-114H

Janelia Fluor 525

NB200-114JF525

Janelia Fluor 549

NB200-114JF549

Janelia Fluor 585

NB200-114JF585

Janelia Fluor 635

NB200-114JF635

Janelia Fluor 646

NB200-114JF646

Janelia Fluor 669

NB200-114JF669

mFluor Violet 450 SE

NB200-114MFV450

mFluor Violet 500 SE

NB200-114MFV500

mFluor Violet 610 SE

NB200-114MFV610

Forumulation

Catalog #

BSA Free

NB200-114

Azide and BSA Free

NBP2-80854

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 8B4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-2 Antibodies »

Product Summary for MMP-2 Antibody (8B4) [CoraFluor™ 1]

Immunogen

Activated recombinant human MMP-2.

Specificity

This is specific for pro and active MMP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for MMP-2 Antibody (8B4) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: MMP-2

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. MMP2 triggers cells to begin migration by cleaving laminin-5 and exposing an integrin-binding site on the epithelial basement membrane. The cleaved of laminin has been detected in tumors and in tissues being altered, howver the activated form of MMP2 is not found in benign tumors. Thus, detection of MMP2 is a possible early indicator of tumor activity. MMP2 is most strongly expressed in normal skin fibroblasts, but may also be found in gliomas, breast and prostate tumors. Mutations in the MMP-2 gene have been linked to Torg-Winchester syndrome, Nodulosis-Arthropathy-Osteolysis (NAO) syndrome, and arthritis. Additionally, abnormal expression of matrix matalloproteinases have been associated with neoplastic traits such as loss of negative growth regulation and high invasive potential.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for MMP-2 Antibody (8B4) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MMP-2 Antibody (8B4) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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