MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80854

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8B4

Format

Azide and BSA Free

Concentration

1 mg/ml

View all MMP-2 Antibodies »

Product Specifications

Immunogen

Activated recombinant human MMP-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

Western Blot: MMP-2 Antibody (8B4)(Pro and Active)Azide and BSA Free [NBP2-80854]

Western Blot: MMP-2 Antibody (8B4)(Pro and Active)Azide and BSA Free [NBP2-80854]

Western Blot: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854] - Analysis of MMP2 expression in ProMMP2 (1), Active MMP2 (2) and human pressure ulcer biopsy (3). Image from the standard format of this antibody.
Immunohistochemistry: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854]

Immunohistochemistry: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854]

Immunohistochemistry: MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free [NBP2-80854] - Detection of MMP-2 staining in FFPE human ovary tissue. Primary antibody was used at 2.5 ug/ml with the avidin-biotin-peroxidase detection method. Image from the standard format of this antibody.

Applications for MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.03 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Frozen

2ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-2ug/ml
Application Notes
In Western blot, a band is seen at 70 and 62kDa representing the MMP2 band-pair. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MMP-2

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. The MMP2 antibody clone MMP2/8B4 was created against activated recombinant human MMP2, and will specifcally detect both the active and inactive forms. This MMP-2 antibody has been thoroughly characterized in most common immunological applications and has been cited in a number of published journal articles.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-2 Antibody (8B4) - (Pro and Active) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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