Mouse ACE-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3437
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat, Transgenic Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740
Accession # Q8R0I0
Gln18-Thr740
Accession # Q8R0I0
Specificity
Detects mouse ACE-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat ACE‑2 and 10% cross-reactivity with recombinant human ACE-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse ACE-2 Antibody
Detection of Mouse ACE‑2 by Western Blot.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of ACE-2 in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Mouse ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF3437) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
ACE-2 in Mouse Kidney.
ACE-2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Applications for Mouse ACE-2 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse ACE-2 DuoSet ELISA Kit (Catalog # DY3437-05) for convenient development of a sandwich ELISA.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse ACE-2 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with mouse ACE-2 and eGFP
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney
Sample: Perfusion fixed frozen sections of mouse kidney
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ACE‑2 (Catalog # 3437‑ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ACE‑2 (Catalog # 3437‑ZN), see our available Western blot detection antibodies
Simple Western
2.5 µg/mL
Sample: Mouse kidney tissue
Sample: Mouse kidney tissue
Western Blot
0.25 µg/mL
Sample: Mouse kidney tissue
Sample: Mouse kidney tissue
Reviewed Applications
Read 3 reviews rated 4.7 using AF3437 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ACE-2
ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).
References
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Crackower, M.A. et al. (2002) Nature 417:822.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Kuba, K. et al. (2005) Nature Med. 11:875.
- Ima, Y. et al. (2005) Nature 436:112.
Long Name
Angiotensin I Converting Enzyme 2
Alternate Names
ACE2, ACEH
Entrez Gene IDs
Gene Symbol
ACE2
UniProt
Additional ACE-2 Products
Product Documents for Mouse ACE-2 Antibody
Product Specific Notices for Mouse ACE-2 Antibody
For research use only
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