Mouse alpha2-Macroglobulin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5798

R&D Systems, part of Bio-Techne
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AF5798
AF5798-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all alpha 2-Macroglobulin Antibodies »

Product Specifications

Immunogen

Mouse serum-derived alpha2-Macroglobulin

Specificity

Detects mouse alpha2-Macroglobulin in direct ELISAs and Western blots. In direct ELISA, less than 2% cross-reactivity with human alpha2‑Macroglobulin, recombinant rat (rr)  alpha2-Macroglobulin, and rr beta2-Macroglobulin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse alpha2-Macroglobulin Antibody

Detection of Mouse a2-Macroglobulin antibody by Western Blot.

Detection of Mouse alpha2-Macroglobulin by Western Blot.

Western blot shows samples of purified a2-Macroglobulin from mouse sera. PVDF membrane was probed with 0.1 µg/mL Sheep Anti-Mouse a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5798) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for a2-Macroglobulin was detected at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse alpha2-Macroglobulin Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Purified alpha2-Macroglobulin from mouse sera

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 2-Macroglobulin

alpha2-Macroglobulin (alpha 2 macroglobulin; also A2M and MAM) is a 185 kDa member of the protease inhibitor I39 family of proteins. It is secreted by the liver, circulates at a constant level in the blood, binds multiple growth factors, inhibits a wide variety of proteinases via steric trapping, is cleared by cell surface receptors and likely exists as a tetramer. Mature mouse A2M is 1471 amino acids (aa) in length. It is cleaved between Arg1239 and Asp1240 to generate two subunits, one that is 165 kDa in size and 1215 aa in length and a second that is 35 kDa in size and 256 aa in length. The protease inhibitor region (aa 686-744) is on the large subunit, while the receptor binding segment (aa 1395-1482) is on the smaller subunit. A2M has a counterpart gene termed A2M-P that is associated with pregnancy. The A2M-P molecule shares 56% aa identity with mouse A2M. Notably, mouse A2M-P is closer in aa sequence to human A2M (72%) and rat A2M (91%) than mouse A2M is to human A2M (59%) and rat A2M (56%).

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Entrez Gene IDs

2 (Human)

Gene Symbol

A2M

Additional alpha 2-Macroglobulin Products

Product Documents for Mouse alpha2-Macroglobulin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse alpha2-Macroglobulin Antibody

For research use only

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