Mouse Amnionless Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7139
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Amnionless
Ala20-Gly362
Accession # Q99JB7
Ala20-Gly362
Accession # Q99JB7
Specificity
Detects mouse Amnionless in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Amnionless is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Amnionless Antibody
Detection of Mouse Amnionless by Western Blot.
Western blot shows recombinant mouse Amnionless (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Amnionless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7139) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Amnionless at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Amnionless in Mouse Kidney.
Amnionless was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Amnionless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7139) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse Amnionless Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney
Sample: Perfusion fixed frozen sections of mouse kidney
Western Blot
1 µg/mL
Sample: Recombinant Mouse Amnionless
Sample: Recombinant Mouse Amnionless
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Amnionless
AMN (Amnionless) is a 45-49 kDa glycoprotein that is expressed by three cell types in mouse; extraembryonic visceral endoderm, proximal tubule renal epithelium, and absorption intestinal epithelium. AMN is required for gastrulation in rodent (but not human), and participates in Vitamin B12 absorption in the gut. In particular, AMN forms a membrane complex with Cubilin, promoting its proper orientation in the membrane and subsequent binding to the Intrinsic factor: VitB12 complex. Mature mouse AMN is a 439 amino acid (aa) type I transmembrane protein. It contains a 343 aa extracellular region (aa 20-362) that possesses one VWFC domain (aa 203‑254), and a 75 aa cytoplasmic tail. In human, AMN may undergo cleavage to generate a 35 kDa soluble fragment. Over aa 20-362, mouse AMN shares 92% and 67% aa sequence identity with rat and human AMN, respectively.
Additional Amnionless Products
Product Documents for Mouse Amnionless Antibody
Product Specific Notices for Mouse Amnionless Antibody
For research use only
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