Mouse BACE-2 Ectodomain Antibody

Name: Mouse BACE-2 Ectodomain Antibody
Brand: R&D Systems
SKU: AF2977

R&D Systems, part of Bio-Techne | Catalog # AF2977

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all BACE-2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BACE-2
Phe29-Pro462 (Tyr357His)
Accession # Q9JL18

Specificity

Detects mouse BACE-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse BACE and recombinant human BACE-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse BACE-2 Ectodomain Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse BACE‑2 (Catalog # 2977‑AS), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse BACE‑2 (Catalog # 2977‑AS)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACE-2

BACE-2 is an aspartic protease that shares sequence similarity with BACE-1, the major beta-secretase for generation of A beta peptide in neurons (1). However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production (2‑4). The domain structure of mouse BACE-2 consists of a signal peptide (aa 1‑19), pro (aa 20‑62) and a mature chain (aa 63‑514) that consists of a transmembrane domain and a cytoplasmic tail. The C-terminal region (aa 463‑514) was replaced by a His tag in the recombinant mouse BACE-2 that also contained a His residue at the position of Tyr357. The purified recombinant mouse BACE-2 started at Ala63, the same residue shown at the N-terminus of the human active BACE-2 (2).

References

Cai, H. et al. (2001) Nature Neurosci. 4:233.
Hussain, I. et al. (2001) J. Biol. Chem. 276:23322.
Ostermann, N. et al. (2006) J. Mol. Biol. 355:249.
Sun, X. et al. (2005) FASEB J. 19:739.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Alternate Names

BACE2

Entrez Gene IDs

25825 (Human); 56175 (Mouse); 288227 (Rat)

Gene Symbol