Mouse Bcl-2 Minus C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB810
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 121529
Product Specifications
Immunogen
E. coli-derived recombinant mouse Bcl-2
Specificity
Detects mouse Bcl‑2 Minus C-Terminus in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Bcl-2 Minus C-Terminus Antibody
Detection of Mouse Bcl-2 by Western Blot.
Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl-2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Mouse Bcl-2 Minus C-Terminus Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: CTLL-2 mouse cytotoxic T cell line
Sample: CTLL-2 mouse cytotoxic T cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-2
Long Name
B Cell Lymphoma/Leukemia 2
Alternate Names
Bcl2
Gene Symbol
BCL2
Additional Bcl-2 Products
Product Documents for Mouse Bcl-2 Minus C-Terminus Antibody
Product Specific Notices for Mouse Bcl-2 Minus C-Terminus Antibody
For research use only
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