Mouse BTN1A1/Butyrophilin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4765
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BTN1A1/Butyrophilin
Ala27-Trp247
Accession # Q62556
Ala27-Trp247
Accession # Q62556
Specificity
Detects mouse BTN1A1/Butyrophilin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human BTN1A1/Butyrophilin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse BTN1A1/Butyrophilin Antibody
Detection of Mouse BTN1A1/Butyrophilin by Western Blot.
Western blot shows lysates of mouse lactating mammary tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse BTN1A1/Butyrophilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4765) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN1A1/Butyrophilin at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse BTN1A1/Butyrophilin Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: Mouse lactating mammary tissue
Sample: Mouse lactating mammary tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4765 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTN1A1/Butyrophilin
Butyrophilin (Greek for “being like butter”; also Btn1a1) is a 66 kDa member of the BTN/MOG family of proteins. It is expressed on secretory mammary epithelium and associates with xanthine dehydrogenase. This is necessary for milk-lipid droplet secretion. Mature mouse Butyrophilin is 498 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 221 aa extracellular domain (ECD) (aa 27‑247) and a 256 aa cytoplasmic region. The ECD contains two V-type Ig‑like domains (aa 29‑141 and aa 149‑235). There are two potential splice variants. One shows a deletion of aa 145‑238, while a second shows a 36 aa substitution for aa 144‑524. Over aa 27‑247, mouse Butyrophilin shares 85% and 67% aa identity with rat and human Butyrophilin, respectively.
Alternate Names
BTN, BTN1A1
Gene Symbol
BTN1A1
UniProt
Additional BTN1A1/Butyrophilin Products
Product Documents for Mouse BTN1A1/Butyrophilin Antibody
Product Specific Notices for Mouse BTN1A1/Butyrophilin Antibody
For research use only
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