Mouse Carboxylesterase 1/CES1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7929
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 1/CES1
His19-Leu565
Accession # Q8VCC2
His19-Leu565
Accession # Q8VCC2
Specificity
Detects mouse Carboxylesterase 1/CES1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CES3 and recombinant human CES1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Carboxylesterase 1/CES1 Antibody
Detection of Mouse Carboxylesterase 1/ CES1 by Western Blot.
Western blot shows lysates of mouse liver. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7929) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxylesterase 1/CES1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Carboxylesterase 1/CES1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse liver
Sample: Mouse liver
Reviewed Applications
Read 1 review rated 5 using AF7929 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxylesterase 1/CES1
Carboxylesterase 1/CES1 (also known as Ses-1 and Liver carboxylesterase 1) is a 60-63 kDa glycoprotein, member of a type-B carboxylesterase/lipase family localized to microsomal membrane and lumen of endoplasmic reticulum. CES1 exists as a homotrimer and homohexamer and is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES1 is expressed mostly in liver, and with lower levels found in the lung. Over amino acids (aa) 19-565, mouse CES1 shares 75% aa identity with human CES1.
Alternate Names
ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH
Gene Symbol
CES1
UniProt
Additional Carboxylesterase 1/CES1 Products
Product Documents for Mouse Carboxylesterase 1/CES1 Antibody
Product Specific Notices for Mouse Carboxylesterase 1/CES1 Antibody
For research use only
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