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Mouse CCL5/RANTES Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4781

R&D Systems, part of Bio-Techne
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MAB4781-100
MAB4781-500
MAB4781-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture, ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 53433

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL5/RANTES
Ser24-Ser91
Accession # P30882

Specificity

Detects recombinant mouse (rm)CCL5 direct ELISAs. In sandwich ELISAs, approximately 5% cross-reactivity with rhCCL5 and less than 0.2% cross-reactivity with rhTECK and rhVIC was observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse CCL5/RANTES Antibody

Application
Recommended Usage

Mouse CCL5/RANTES Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL5/RANTES Biotinylated Antibody (Catalog # BAF478)
  • Standard: Recombinant Mouse CCL5/RANTES Protein (Catalog # 478-MR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3, and 5.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

UniProt

Additional CCL5/RANTES Products

Product Documents for Mouse CCL5/RANTES Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCL5/RANTES Antibody

For research use only

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