Detects mouse CCL6/C10 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 21, 22, 23, 24, 25, 28, recombinant mouse CCL3, 4, 9, 11, 12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL6/C10 Antibody

Chemotaxis Induced by CCL6/C10 and Neutral-ization by Mouse CCL6/C10 Antibody.

Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemo-taxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND₅₀ is typically 6-24 µg/mL.

Applications for Mouse CCL6/C10 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse bone marrow macrophages fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL6/C10 (Catalog # 487-C) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CCL6/C10-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Mouse CCL6/C10.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL6/C10

CCL6, also known as C10 and SCYA6, is a beta chemokine with an extended N-terminus. CCL6 expression is induced by GM-CSF stimulation of monocytes and neutrophils and IL-4 stimulation of macrophages. CCL6 functions as a monocyte and macrophage chemoattractant.

Alternate Names

C10, MRP-1, SCYA6

Entrez Gene IDs

20305 (Mouse); 287910 (Rat)

Gene Symbol

CCL6

UniProt

P27784

Additional CCL6/C10 Products

Product Documents for Mouse CCL6/C10 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCL6/C10 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CCL6/C10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB487

Key Product Details

Species Reactivity

Validated:

Cited:

Applications