Mouse CCR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5986
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCR1
Met1-Ala34, Asp92-Lys107, Phe172-Arg197, Gln265-Leu281
Accession # AAH11092
Met1-Ala34, Asp92-Lys107, Phe172-Arg197, Gln265-Leu281
Accession # AAH11092
Specificity
Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) CCR4, rmCCR5, and rmCCR8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CCR1 Antibody
Detection of Mouse CCR1 by Western Blot.
Western blot shows lysates of mouse heart tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse CCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5986) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse CCR1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse heart tissue and mouse spleen tissue
Sample: Mouse heart tissue and mouse spleen tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR1
CCR1 (C-C chemokine receptor 1; also MIP-1 alpha receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1 alpha/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent agonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C‑terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively.
Alternate Names
CCR1, CD191
Gene Symbol
CCR1
UniProt
Additional CCR1 Products
Product Documents for Mouse CCR1 Antibody
Product Specific Notices for Mouse CCR1 Antibody
For research use only
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